Principles of DNA sequencing, PCR, and genetic testing in clinical laboratories.
Course overview
This course covers the core principles and techniques used in modern molecular diagnostics within clinical laboratories. You will explore the fundamentals of DNA sequencing, polymerase chain reaction (PCR), and genetic testing. The curriculum emphasizes both the theoretical background and the practical application of these methods in a diagnostic setting.
Topics include nucleic acid extraction, amplification strategies, hybridization techniques, and the interpretation of complex genetic data. You will also examine the role of molecular diagnostics in identifying hereditary conditions, infectious diseases, and cancer biomarkers. The course focuses on quality assurance, regulatory compliance, and the clinical relevance of molecular test results.
Who takes this course
This course is an elective for students in the Medical Laboratory Technology program.
Credits: 3 · Hours: 45
Prerequisites
None
Learning outcomes
- Explain the theoretical basis and laboratory workflow for polymerase chain reaction (PCR) and reverse transcription PCR.
- Perform DNA extraction and quantification from clinical samples using standard laboratory protocols.
- Interpret gel electrophoresis and capillary electrophoresis results to identify genetic markers.
- Analyze Sanger sequencing data to identify mutations and variants associated with hereditary conditions.
- Evaluate the clinical validity and utility of molecular tests for diagnostic purposes.
- Apply quality assurance and quality control principles to molecular diagnostic assays.
Evaluation
| Component | Weight (%) |
| Practical Laboratory Reports | 40 |
| Midterm Examination | 25 |
| Final Examination | 35 |